Collagen type II alpha 1 chain Rabbit Polyclonal Antibody

CAT#: BP8009

Collagen type II alpha 1 chain rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: RIA.
  ELISA.
  Indirect Immunofluorescence: 1/40 on frozen tissues.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 1/200-1/600.
  Recommended Incubation Time: 60 min at room temperature or overnight at 2-8°C.
  Recommended Positive Control: Chicken cartilage.
  Pretreatment: After removing paraffin pre-treat with 0.2 % Hyaluronidase (approx. 300 U/mg) in TBS, 15 min at 37°C, then block unspecific binding with blocking serum or 3% BSA in TBS and perform blocking of endogen Peroxidase with 1% H2O2 in TBS, if necessary.
• Reactivities: Chicken
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified collagen type II from fetal chicken cartilage
• Specificity: This antibody is specific for Pro-Collagen Type III.
  Chicken collagen type I: 100%.
  Chicken collagen type II and III: < 0.1%.
  Chicken collagen type X: < 1%.
• Formulation: PBS without BSA or preservatives
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 0.5 ml distilled water, for further dilution use appropriate antibody diluent.
• Concentration: ~1 mg/ml
• Purification: Affinity Chromatography
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type II collagen is an alpha1(II)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and various tumours.
• Synonyms: COL2A1, Alpha-1 type II collagen