Col2a1 Rabbit Polyclonal Antibody

CAT#: BP8010

Col2a1 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: RIA.
  ELISA: 1/100-1/200 (OD ≥ 500).
  Immunofluorescence Assays: 1/40.
  Immunohistochemistry on Frozen and Paraffin Sections: 1/100-1/500.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
  Incubation Time: 60 min at RT or 2-8°C over night.
  Positive Control: Rat Cartilage.
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified Collagen type II from fetal Rat cartilage
• Specificity: This antibody is specific for Rat Collagen type II (100%).
  Cross Reactions:
  Rat Collagen I, III and V: < 0.1%
  Rat Elastin, Rat-Fibronectin: < 0.1% (solid phase RIA at 1/100 dilution).
• Formulation: State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 0.5 ml distilled water.
  For further dilution use appropriate antibody diluent.
• Concentration: ≥ 1.0 mg/ml
• Purification: Ion Exchange Chromatography
• Gene Name: Rattus norvegicus collagen type II alpha 1 chain (Col2a1)
• Database Link:
  Entrez Gene 25412 Rat
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type II Collagen is an alpha1(II)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and various tumours.
• Synonyms: COL2A1, Alpha-1 type II collagen