Col2a1 Rabbit Polyclonal Antibody

CAT#: BP8011S

Col2a1 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: ELISA: 1/200 (OD ≥ 500).
  Radioimmunoassay.
  Immunofluorescence: 1/80.
  Immunohistochemistry on Parrafin Sections: 1/1000 (60 min at RT or 2-8°C over night).
  Positive Control: Mouse Cartilage.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified Collagen type II from Fetal Murine Cartilage.
• Specificity: Murine Procollagen and Collagen type II: 100%.
  Murine Collagen type I, III and IV < 0.1%.
  Mouse Fibronectin: < 0.1%.
  Cross-reacts with Bovine Collagen type II in ELISA.
• Formulation: PBS without BSA and preservatives
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 0.5 ml distilled water, for further dilution use appropriate antibody diluent.
• Concentration: ~1.0 mg/ml
• Purification: Affinity Chromatography
• Gene Name: Mus musculus collagen, type II, alpha 1 (Col2a1), transcript variant 2
• Database Link:
  Entrez Gene 12824 Mouse
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type II collagen is an alpha1(II)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and various tumours.
• Synonyms: COL2A1, Alpha-1 type II collagen