Col3a1 Rabbit Polyclonal Antibody

CAT#: BP8014

Col3a1 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R, WB
• Recommended Dilution: RIA.
  Western blot.
  ELISA: ≥ 1/200.
  Immunflourescence: 1/80.
  Immunohistochemistry on Frozen and Paraffin Embedded Tissues: ≥ 1/500.
  Pretreatment: After de-waxing the tissue slices are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
  Incubation Time: 60 minutes at RT or 2-8°C over night.
  Positive Control: Rat skin or liver.
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified Collagen type III from Rat skin.
• Specificity: Rat Collagen type III: 100%.
  Rat Collagen I: < 0.1%
  Rat Collagen type V: < 10%
  Rat Elastin: < 0.1% (determined by solid phase RIA at 1/50 dilution).
• Formulation: PBS without BSA or preservatives
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 0.5 ml distilled water.
  For further dilutions use appropriate antibody diluent.
• Concentration: ~1.0 mg/ml
• Purification: Affinity Chromatography
• Database Link:
  Entrez Gene 12825 Mouse
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type III collagen is an alpha1(III)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body.
• Synonyms: COL3A1