Collagen type IV Rabbit Polyclonal Antibody

CAT#: BP8015

Collagen type IV rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: The antibody can be used on frozen and paraffin embedded sections.
  IFA: 1:40.
  Immunohistochemistry on paraffin sections: 1:100 - 1:500 (immunoperoxidase, Streptavidin/Biotin, ABC).
  Incubation time: 60 min at RT or 2-8°C over night.
  Positive control:
  Bovine skin or lens capsule.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
  ELISA.
  RIA.
  Immunoflourescence.
• Reactivities: Bovine, Porcine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified collagen type IV from bovine lens capsule
• Specificity: Bovine collagen type IV 100%; bovine collagen I, II, III, IX, XI and bovine fibronectin negativ in ELISA at 1:100 dilution.
• Formulation: State: Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: Reconstitute with 0.5 ml distilled water, for further dilution use appropriate antibody diluent
• Concentration: 1.0 mg/ml
• Purification: Ion exchange chromatography
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type IV Collagen (170-180 kDa), is a non-fibrilary network of different alpha-chains. It is typically found in basal membranes of different organs (e.g. skin, lens, lung, renals).
• Synonyms: COL4A1