Col4a1 Rabbit Polyclonal Antibody

CAT#: BP8016

Col4a1 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: RIA.
  ELISA: ≥ 1/200 (OD = 0.5).
  Immunofluorescence: 1/80.
  Immunohistochemistry on Paraffin Sections: 1/200-1/600.
  Incubation Time: 60 min at RT or 2-8°C over night.
  Positive Control: Rat skin or Liver.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified Collagen type IV from ruminant tumour tissue
• Specificity: Rat Collagen type IV: 100%
  Rat Collagen type I, II, III and V: < 0.1%
  Human Collagen type IV: < 0.1%
  Rat and Murine Fibronectin: < 0.1%
  Murine Laminin: < 0.1% (RIA at 1/5000 dilution).
• Formulation: PBS without BSA or preservatives.
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 0.5 ml distilled water.
  For further dilutions use appropriate antibody diluent.
• Concentration: ~1.0 mg/ml
• Purification: Affinity Chromatography.
• Gene Name: Mus musculus collagen, type IV, alpha 1 (Col4a1), transcript variant 1
• Database Link:
  Entrez Gene 290905 RatEntrez Gene 12826 Mouse
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type IV Collagen (170-180 kDa), is a non-fibrilary network of different alpha-chains. It is typically found in basal membranes of different organs (e.g. skin, lens, lung, renals).
• Synonyms: COL4A1