Col5a1 Rabbit Polyclonal Antibody

CAT#: BP8020

Col5a1 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: RIA.
  ELISA: > 1/100-1/200 (OD > 500).
  Immunofluorescence: 1/40.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Embedded Tissues: 1/200-1/600.
  Incubation time: 60 min. at RT or 2-8°C overnight.
  Positive Control: Rat skin or liver.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Reactivities: Bovine, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified collagen type V from Rat skin.
• Specificity: Rat Collagen type V: 100%
  Rat Collagen type I, II and II: < 0.1%
  Rat Elastin: < 0.1% (RIA at 1/100 dilution).
• Formulation: PBS without BSA and preservatives.
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 0.5 ml distilled water.
  For further dilution use appropriate antibody diluent.
• Concentration: ~1.0 mg/ml
• Purification: Ion Exchange Chromatography
• Gene Name: Rattus norvegicus collagen type V alpha 1 chain (Col5a1)
• Database Link:
  Entrez Gene 12831 MouseEntrez Gene 85490 Rat
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Collagen type V is found as short fibrils in skin, bone and placenta. Often it is found in conjunction with other collagen types, especially type I and III.
• Synonyms: COL5A1