Eln Rabbit Polyclonal Antibody

CAT#: BP8022

Eln rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R, WB
• Recommended Dilution: RadioImmunoAssay.
  Immunofluorescence: 1/40.
  ELISA: ≥ 1/200.
  Immunoblotting (Western blot).
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: 1/500 (immunoperoxidase, Streptavidin/Biotin, ABC).
  Incubation time: 60 min at RT or 2-8°C overnight.
  Pretreatment: After de-waxing the tissue slices are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
  Positive Control: Rat skin or aorta.
• Reactivities: Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified Elastin from Rat Aorta.
• Specificity: Rat Elastin 100%
  Rat Collagen type I, II, III and V < 0.1% (RIA at 1/500 dilution).
• Formulation: PBS without preservatives or stabilizers
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 0.5 ml distilled water.
  Dilute further with appropriate antibody diluent.
• Concentration: ~1.0 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Database Link:
  Entrez Gene 25043 Rat
• Background: Elastin is the major protein of elastic fibres that form a randomly orientated network in many tissues. Due to its high content of hydrophobic amino acids it is relatively resistant to chemicals and proteinase. Its principal function is to provide elasticity to tissues, however it has also chemotactic and cell adhesion properties.
• Synonyms: ELN, Tropoelastin