Eln Rabbit Polyclonal Antibody

CAT#: BP8022

Eln rabbit polyclonal antibody, Purified


USD 970.00

In Stock*

Size
    • 500 ul

Product Images

Specifications

Product Data
Applications ELISA, IF, IHC, R, WB
Recommended Dilution RadioImmunoAssay.
Immunofluorescence:
1/40.
ELISA: ≥ 1/200.
Immunoblotting (Western blot).
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections:
1/500 (immunoperoxidase, Streptavidin/Biotin, ABC).
Incubation time: 60 min at RT or 2-8°C overnight.
Pretreatment: After de-waxing the tissue slices are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Positive Control: Rat skin or aorta.
Reactivities Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen Purified Elastin from Rat Aorta.
Specificity Rat Elastin 100%
Rat Collagen type I, II, III and V < 0.1% (RIA at 1/500 dilution).
Formulation PBS without preservatives or stabilizers
State: Purified
State: Lyophilized purified IgG fraction
Reconstitution Method Restore with 0.5 ml distilled water.
Dilute further with appropriate antibody diluent.
Concentration ~1.0 mg/ml (after reconstitution)
Purification Affinity Chromatography
Background Elastin is the major protein of elastic fibres that form a randomly orientated network in many tissues. Due to its high content of hydrophobic amino acids it is relatively resistant to chemicals and proteinase. Its principal function is to provide elasticity to tissues, however it has also chemotactic and cell adhesion properties.
Synonyms ELN, Tropoelastin
Reference Data

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