Fn1 Rabbit Polyclonal Antibody

CAT#: BP8025

Fn1 rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IHC, R
• Recommended Dilution: Immunohistochemistry on Paraffin Sections: 1/200-1/600.
  Immunofluorescence: 1/80.
  Incubation Time: 60 min at RT or 2-8°C over night.
  Positive Control: Rat Skin, Liver.
  Pretreatment: After de-waxing the tissue slices are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
  Also suitable for use in RIA and ELISA: >/= 1/200 (OD >/=500).
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified Fibronectin from Rat plasma.
• Specificity: Cross reactivity:
  Rat Fibronectin: 100%,
  Rat Collagen type I, III and IV: < 0.1%,
  Rat Elastin: < 0.1% (RIA at 1/5000 dilution).
  This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.
• Formulation: PBS without BSA or preservatives.
  State: Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 0.5 ml distilled water.
• Concentration: ~1.0 mg/ml
• Purification: Affinity Chromatography.
• Gene Name: fibronectin 1
• Database Link:
  Entrez Gene 25661 Rat
• Background: Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumors.
• Synonyms: FN1, Cold-insoluble globulin, CIG