Collagen I (COL1A1) Rabbit Polyclonal Antibody

CAT#: BP8028

Collagen I (COL1A1) rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: RIA.
  ELISA: 1/200 (OD ≥ 500).
  Immunflourescence Assays: 1/80.
  Immunohistochemistry on Paraffin Sections: 1/200-1/600.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% Hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
  Incubation Time: 60 min at RT or 2-8°C over night.
  Positive Control: Human skin.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified Collagen type I from Human skin.
• Specificity: Human Collagen type I: 100%
  Collagen type II and IV: < 0.1%
  Collagen type III and V: < 1.0%
  Human Fibronectin: < 0.1%.
• Formulation: Phosphate buffered solution, no BSA and preservative added.
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 0.1 ml (Cat.-No BP8028S) or 0.5 ml (Cat.-No BP8028) distilled water.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens collagen type I alpha 1 chain (COL1A1)
• Database Link:
  Entrez Gene 1277 Human
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.
• Synonyms: COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: ECM-receptor interaction, Focal adhesion