Collagen II (COL2A1) Rabbit Polyclonal Antibody

CAT#: BP8029

Collagen II (COL2A1) rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: ELISA.
  RIA.
  Immunofluorescence: 1/40.
  Immunohistochemistry: 1/200-1/600 for Paraffin Sections, 60 min at RT or 2-8°C over night.
  Positive Control: Human skin.
  Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified Collagen type II from Human skin.
• Specificity: This antibody is specific for Collagen type II.
  Cross-reactivity:
  Human Collagen type I and IV: < 1%
  Human Collagen type III, IX and XI: < 0.1%.
  Human Fibronectin: < 0.1% (RIA: 1/200).
• Formulation: PBS without additives.
  State: Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 0.5 ml distilled water, for further dilution use appropriate antibody diluent.
• Concentration: lot specific
• Purification: Affinity Chromatography.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens collagen type II alpha 1 chain (COL2A1), transcript variant 1
• Database Link:
  Entrez Gene 1280 Human
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type II collagen is an alpha1(II)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and various tumours.
• Synonyms: COL2A1, Alpha-1 type II collagen

Reference Data

• Protein Pathways: ECM-receptor interaction, Focal adhesion