Procollagen Type III Rabbit Polyclonal Antibody

CAT#: BP8034S

Procollagen Type III rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, R
• Recommended Dilution: RIA: > 1/200.
  ELISA: 1/100-1/200 (OD ≥ 500).
  Immunofluorescence (indirect, 1/40 on Frozen tissues).
  Immunohistochemistry on Frozen and Paraffin Embedded Tissue Sections: 1/500.
  Pretreatment: After removing Paraffin pre-treat with 0.2% Hyaluronidase (approx. 300 U/mg) in TBS, 15 min at 37°C, then block unspecific binding with blocking serum or 3% BSA in TBS and perform blocking of endogen Peroxidase with 1% H2O2 in TBS, if necessary.
  Incubation Time: 60 min at room temperature or overnight at 2-8°C.
  Positive Control: Human or Bovine Skin and Liver.
• Reactivities: Bovine, Human, Porcine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified Human Procollagen type III C-terminal and N-terminal PIIICP separated.
• Specificity: Human and Bovine Pro-Collagen Type III (100%).
  Human and Bovine Pro-Collagen Typ I and Collagen Typ I <0.1% (RIA at 1/200 dilution).
• Formulation: PBS without BSA or preservatives
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 0.1 ml sterile distilled water.
• Concentration: ~1.0 mg/ml (after reconstitution)
• Purification: Affinity Chromatography
• Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
  Type III collagen is an alpha1(III)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body.
• Synonyms: Collagen, Pro-Collagen