Procollagen Type III Rabbit Polyclonal Antibody

CAT#: BP8034S

Procollagen Type III rabbit polyclonal antibody, Purified


USD 490.00

2 Weeks*

Size
    • 100 ul

Product Images

Specifications

Product Data
Applications ELISA, IF, IHC, R
Recommended Dilution RIA: > 1/200.
ELISA: 1/100-1/200 (OD ≥ 500). 
Immunofluorescence (indirect, 1/40 on Frozen tissues).
Immunohistochemistry on Frozen and Paraffin Embedded Tissue Sections: 1/500.
Pretreatment: After removing Paraffin pre-treat with 0.2% Hyaluronidase (approx. 300 U/mg) in TBS, 15 min at 37°C, then block unspecific binding with blocking serum or 3% BSA in TBS and perform blocking of endogen Peroxidase with 1% H2O2 in TBS, if necessary.
Incubation Time: 60 min at room temperature or overnight at 2-8°C.
Positive Control: Human or Bovine Skin and Liver.
Reactivities Bovine, Human, Porcine
Host Rabbit
Clonality Polyclonal
Immunogen Purified Human Procollagen type III C-terminal and N-terminal PIIICP separated.
Specificity Human and Bovine Pro-Collagen Type III (100%).
Human and Bovine Pro-Collagen Typ I and Collagen Typ I <0.1% (RIA at 1/200 dilution).
Formulation PBS without BSA or preservatives
State: Purified
State: Lyophilized purified Ig fraction
Reconstitution Method Restore with 0.1 ml sterile distilled water.
Concentration ~1.0 mg/ml (after reconstitution)
Purification Affinity Chromatography
Background Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs.
Type III collagen is an alpha1(III)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body.
Synonyms Collagen, Pro-Collagen
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.