RNA5-8SN2 Mouse Monoclonal Antibody [Clone ID: OTI3F7]

CAT#: CF190077

Carrier-free (BSA/glycerol-free) NF mouse monoclonal antibody,clone OTI3F7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Neurofilament ([RC000000], Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Neurofilament (1:500).

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  Western blot analysis of extracts (35ug) from 6 different cell lines and 2 different tissue lysates by using anti-Neurofilament monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human adult heart tissue within the normal limits using anti-Neurofilament mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA190077) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-Neurofilament mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA190077) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-Neurofilament mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA190077) (1:2000)

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Specifications

Product Data

• Clone Name: OTI3F7
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide: EYQDLLNVKMALDIEIAAYRKLLEGEE, corresponding to the conserved region of human neurofilament heavy polypeptide (NEFH), medium polypeptide (NEFM) and light polypeptide (NF68, NEFL)
• Formulation: PBS (pH7.3), 8% Trehalose
• Reconstitution Method: Reconstitute with PBS (pH7.3). To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids by affinity chromatography
• Gene Name: Homo sapiens RNA, 5.8S ribosomal N2 (RNA5-8SN2), ribosomal RNA.
• Synonyms: RNA5-8N2