Rat Monoclonal Antibody [Clone ID: OTI3E12]

CAT#: CF190113

Carrier-free (BSA/glycerol-free) IDH1-mutant rat monoclonal antibody, clone OTI3E12 (formerly 3E12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane "293T") or pCMV6-ENTRY IDH1 (wild type RC210582, middle lane, ) or pCMV6-ENTRY IDH1 mutated ( R132H mutation RC400096, right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (10 ug per lane) were separated by SDS-PAGE and immunoblotted with TA190113 (1:500) and then goat anti-rat IgG-HRP (1:2000).

Specifications

Product Data

• Clone Name: OTI3E12
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Rat
• Clonality: Monoclonal
• Immunogen: Synthetic peptide around the R132H mutation region of the human IDH conjugated to KLH
• Formulation: PBS (pH7.3), 8% Trehalose
• Reconstitution Method: Reconstitute with PBS (pH7.3). To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from cell culture supernatant by affinity chromatography