Rat Monoclonal Antibody [Clone ID: OTI1D10]

CAT#: CF190116

Carrier-free (glycerol/BSA-free) Acidic CK rat monoclonal antibody, clone OTI1D10 (formerly 1D10)

Product Images

HEK293T cells were transfected with 55 different plasmids of pCMV6-ENTRY with CK (1, 2, 4, 5, 6a, 6b, 6c, 7, 8, 9, 12, 13, 14, 15, 16, 17, 18 v1, 18 v2, 19, 20, 24, 25, 26, 27, 28, 31, 32, 33a, 33b, 34, 35, 36, 37, 38, 39, 40, 71, 72 v1, 72 v3, 73, 74, 75, 76, 77, 78, 79, 80 v1, 80 v2, 81, 82, 83, 84, 85, 86 and 222) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CK antibody. As shown in the figure, KRT12, 15, 16, 17, 19, 24, 25, 26, 27, 28, 37 and 39 were positive, while all the others were negative (1:500).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Acidic CK rat monoclonal antibody. (TA190116)

• Immunohistochemical staining of paraffin-embedded Human tonsil using anti-Acidic CK rat monoclonal antibody. (TA190116)

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Specifications

Product Data

• Clone Name: OTI1D10
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human
• Host: Rat
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Synthetic peptide: EKVTMQNLND RLASYLDKVR ALEEAN, corresponding to the conserved region of human type I (Acidic) keratins (including KRT9, KRT10, KRT12, KRT13, KRT14, KRT15, KRT16, KRT17, KRT18, KRT19, KRT20).
• Formulation: PBS (pH7.3), 8% Trehalose
• Reconstitution Method: Reconstitute with PBS (pH7.3). To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from cell culture supernatant by affinity chromatography