PKM2 Mouse Monoclonal Antibody [Clone ID: OTI12D5]

CAT#: CF190266

Carrier-free (BSA/glycerol-free) PKM2 mouse monoclonal antibody, clone OTI12D5 (formerly 12D5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKM2 (RC201855, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKM2.

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-PKM2 monoclonal antibody at 1:200 (1: SVT2; 2: MDCK; 3: PC12).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190266)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PKM2 mouse monoclonal antibody. (TA190266)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190266)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PKM2 mouse monoclonal antibody. (TA190266)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190266)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PKM2 mouse monoclonal antibody. (TA190266)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190266)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PKM2 mouse monoclonal antibody. (TA190266)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190266)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PKM2 mouse monoclonal antibody. (TA190266)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190266)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PKM2 mouse monoclonal antibody. (TA190266)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190266)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PKM2 mouse monoclonal antibody. (TA190266)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190266)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PKM2 mouse monoclonal antibody. (TA190266)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190266)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PKM2 mouse monoclonal antibody. (TA190266)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190266)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PKM2 mouse monoclonal antibody. (TA190266)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PKM2 mouse monoclonal antibody. (TA190266)

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Specifications

Product Data

• Clone Name: OTI12D5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Synthetic peptide: LRRLAPITSDPTEATAVGAV, corresponding to the region encoded by PKM gene M2 isoform.
• Formulation: PBS (pH7.3), 8% Trehalose
• Reconstitution Method: Reconstitute with PBS (pH7.3). To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids by affinity chromatography