PKM2 Mouse Monoclonal Antibody [Clone ID: OTI14E1]

CAT#: CF190268

Carrier-free (BSA/glycerol-free) PKM2 mouse monoclonal antibody, clone OTI14E1 (formerly 14E1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKM2 (RC201855, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKM2.

Specifications

Product Data

• Clone Name: OTI14E1
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide: LRRLAPITSDPTEATAVGAV, corresponding to the region encoded by PKM gene M2 isoform.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.