Mouse Monoclonal Antibody [Clone ID: OTI11G10]

CAT#: CF190269

Carrier-free (BSA/glycerol-free) PKM2 mouse monoclonal antibody, clone OTI11G10 (formerly 11G10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKM2 (RC201855, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKM2.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PKM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA190269)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PKM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA190269)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PKM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA190269)

Specifications

Product Data

• Clone Name: OTI11G10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide: LRRLAPITSDPTEATAVGAV, corresponding to the region encoded by PKM gene M2 isoform.
• Formulation: PBS (pH7.3), 8% Trehalose
• Reconstitution Method: Reconstitute with PBS (pH7.3). To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids by affinity chromatography