STAT4 Mouse Monoclonal Antibody [Clone ID: OTI2A2]

CAT#: CF500005

Carrier-free (BSA/glycerol-free) Stat4 mouse monoclonal antibody, clone OTI2A2 (formerly 2A2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Stat4 (RC206892, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Stat4. Positive lysates LY401095 (100ug) and LC401095 (20ug) can be purchased separately from OriGene.

• 

  STAT4 antibody ( 2A2 ) at 1:100 dilution + Hela cell lysate

• 

  Immunohistochemical staining of paraffin-embedded kidney within the normal limits using anti-Stat4 (TA500005) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-Stat4 (TA500005) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Specifications

Product Data

• Clone Name: OTI2A2
• Applications: IHC, WB
• Recommended Dilution: WB: 1:100, IHC 1:50
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant fragment expressed in E.coli corresponding to amino acids 633-652 of human Stat4
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85.8 kDa
• Gene Name: Homo sapiens signal transducer and activator of transcription 4 (STAT4), transcript variant 1, mRNA.
• Database Link:
  NP_003142
  Entrez Gene 20849 MouseEntrez Gene 367264 RatEntrez Gene 6775 Human
  UniProt ID Q14765
• Background: Stat4 is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.
• Synonyms: SLEB11

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Jak-STAT signaling pathway