Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: OTI5E8]

CAT#: CF500016

Carrier-free (BSA/glycerol-free) CK18 mouse monoclonal antibody, clone OTI5E8 (formerly 5E8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT18 (RC210049, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT18. Positive lysates LY404683 (100ug) and LC404683 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT18 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and KRT18-Knockout HeLa cells (KO, Cat# LC831285) were separated by SDS-PAGE and immunoblotted with anti-KRT18 monoclonal antibody TA500016 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-KRT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500016)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KRT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500016)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KRT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500016)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-KRT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500016)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500016)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-KRT18 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500016)

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Specifications

Product Data

• Clone Name: OTI5E8
• Applications: IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 69-372 of human CK18 (NP_000215) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47.9 kDa
• Gene Name: Homo sapiens keratin 18 (KRT18), transcript variant 2, mRNA.
• Database Link:
  NP_954657
  Entrez Gene 16668 MouseEntrez Gene 294853 RatEntrez Gene 477601 DogEntrez Gene 3875 Human
  UniProt ID P05783
• Background: Keratin 18 together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
• Synonyms: CK-18; CYK18; K18

Reference Data

• Protein Pathways: Pathogenic Escherichia coli infection