Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: OTI1B9]

CAT#: CF500020

Carrier-free (BSA/glycerol-free) CK8 mouse monoclonal antibody, clone OTI1B9 (formerly 1B9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8. Positive lysates LY419425 (100ug) and LC419425 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:500).

• 

  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and KRT8-Knockout Hela cells (KO, Cat# [LC810123]) were separated by SDS-PAGE and immunoblotted with anti-KRT8 monoclonal antibody TA500020, (1:2000. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

• 

  Anti-KRT8 mouse monoclonal antibody (TA500020) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8 (RC209570).

Specifications

Product Data

• Clone Name: OTI1B9
• Applications: IF, WB
• Recommended Dilution: WB 1:2500~5000, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.5 kDa
• Gene Name: Homo sapiens keratin 8 (KRT8), transcript variant 2, mRNA.
• Database Link:
  NP_002264
  Entrez Gene 16691 MouseEntrez Gene 25626 RatEntrez Gene 486513 DogEntrez Gene 3856 Human
  UniProt ID P05787
• Background: Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
• Synonyms: CARD2; CK-8; CK8; CYK8; K2C8; K8; KO

Reference Data

• Protein Families: Druggable Genome