Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: OTI2E4]

CAT#: CF500022

Carrier-free (BSA/glycerol-free) CK8 mouse monoclonal antibody, clone OTI2E4 (formerly 2E4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8. Positive lysates LY419425 (100ug) and LC419425 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500022) (1:750)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500022)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500022) (1:750)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500022) (1:750)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500022) (1:750)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500022) (1:750)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500022) (1:750)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500022) (1:750)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500022) (1:750)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500022) (1:750)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500022) (1:750)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500022) (1:750)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KRT8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500022) (1:750)

• Anti-KRT8 mouse monoclonal antibody (TA500022) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8 (RC209570).

• Immunofluorescent staining of A549 cells using anti-KRT8 mouse monoclonal antibody (TA500022).

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Specifications

Product Data

• Clone Name: OTI2E4
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:50, IF 1:50~100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.5 kDa
• Gene Name: Homo sapiens keratin 8 (KRT8), transcript variant 2, mRNA.
• Database Link:
  NP_002264
  Entrez Gene 16691 MouseEntrez Gene 25626 RatEntrez Gene 3856 Human
  UniProt ID P05787
• Background: Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
• Synonyms: CARD2; CK-8; CK8; CYK8; K2C8; K8; KO

Reference Data

• Protein Families: Druggable Genome