Hex (HHEX) Mouse Monoclonal Antibody [Clone ID: OTI4B7]

CAT#: CF500025

Carrier-free (BSA/glycerol-free) Hex mouse monoclonal antibody, clone OTI4B7 (formerly 4B7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HHex (RC204815, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HHex. Positive lysates LY419139 (100ug) and LC419139 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HHex monoclonal antibody at 1:500 dilution (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Hex antibody ( 4B7 ) at 1:1000 with Lysates from HEK-293T cells transfected with human Hex expression vector (Cat# RC204815)

Specifications

Product Data

• Clone Name: OTI4B7
• Applications: WB
• Recommended Dilution: WB: 1:500 - 1:1000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant fragment expressed in E.coli corresponding to amino acids 100-270 of human Hex
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.8 kDa (experimental 44 kDa)
• Gene Name: Homo sapiens hematopoietically expressed homeobox (HHEX), mRNA.
• Database Link:
  NP_002720
  Entrez Gene 15242 MouseEntrez Gene 79237 RatEntrez Gene 3087 Human
  UniProt ID Q03014
• Background: Hex is a member of the homeobox family of transcription factors, many of which are involved in developmental processes. Expression in specific hematopoietic lineages suggests that this protein may play a role in hematopoietic differentiation.
• Synonyms: HEX; HMPH; HOX11L-PEN; PRH; PRHX

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young