Noggin (NOG) Mouse Monoclonal Antibody [Clone ID: OTI1H8]

CAT#: CF500029

Carrier-free (BSA/glycerol-free) Noggin mouse monoclonal antibody, clone OTI1H8 (formerly 1H8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nog (RC205020, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nog. Positive lysates LY401672 (100ug) and LC401672 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-Nog monoclonal antibody.

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  Noggin antibody (1H8) at 1:40000 + Recombinant human Noggin (Cat# RC205020)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-Nog mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500029)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-Nog mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500029)

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  Anti-Nog mouse monoclonal antibody (TA500029) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY Nog (RC205020).

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Specifications

Product Data

• Clone Name: OTI1H8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:20000~40000, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 28-232 of human noggin (NP_005441) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.7 kDa
• Gene Name: Homo sapiens noggin (NOG), mRNA.
• Database Link:
  NP_005441
  Entrez Gene 18121 MouseEntrez Gene 25495 RatEntrez Gene 9241 Human
  UniProt ID Q13253
• Background: Noggin is a secreted polypeptide which binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, it may have a principal role in creating morphogenic gradients. Noggin appears to have pleiotropic effect, both early in development as well as in later stages. The results of the mouse knockout of the ortholog suggest that noggin is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse.
• Synonyms: SYM1; SYNS1; SYNS1A

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: TGF-beta signaling pathway