EPCAM Mouse Monoclonal Antibody [Clone ID: OTI2H9]

CAT#: CF500056

Carrier-free (BSA/glycerol-free) EpCAM mouse monoclonal antibody, clone OTI2H9 (formerly 2H9)

Product Images

Immunofluorescent staining of HeLa cells using anti-EpCAM mouse monoclonal antibody (TA500056).

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  Anti-EpCAM mouse monoclonal antibody (TA500056) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY EpCAM (RC201989)

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EpCAM (RC201989, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EpCAM. Positive lysates LY400847 (100ug) and LC400847 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-EPCAM monoclonal antibody at 1:200.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-TACSTD1 monoclonal antibody (1:200).

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Specifications

Product Data

• Clone Name: OTI2H9
• Applications: IF, WB
• Recommended Dilution: WB: 1:200 - 1:500, IF: 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 24-265 of human EpCAM
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.7 kDa
• Gene Name: Homo sapiens epithelial cell adhesion molecule (EPCAM), mRNA.
• Database Link:
  NP_002345
  Entrez Gene 4072 Human
  UniProt ID P16422
• Background: EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas
• Synonyms: 4; DIAR5; EGP-2; EGP40; EGP314; ESA; HNPCC8; KS1; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transmembrane