ICAD (DFFA) Mouse Monoclonal Antibody [Clone ID: OTI5C4]

CAT#: CF500062

Carrier-free (BSA/glycerol-free) DFF45 mouse monoclonal antibody, clone OTI5C4 (formerly 5C4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (RC202879, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA. Positive lysates LY418008 (100ug) and LC418008 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DFFA monoclonal antibody.

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  Anti-DFFA mouse monoclonal antibody (TA500062) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DFFA (RC202879).

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  Immunofluorescent staining of A549 cells using anti-DFFA mouse monoclonal antibody (TA500062).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and DFFA-Knockout HeLa cells (KO, Cat# LC833352) were separated by SDS-PAGE and immunoblotted with anti-DFFA monoclonal antibody TA500062 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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Specifications

Product Data

• Clone Name: OTI5C4
• Applications: IF, WB
• Recommended Dilution: WB 1:2500~5000, IF 1:50~100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DFFA (NP_004392) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.3 kDa
• Gene Name: Homo sapiens DNA fragmentation factor subunit alpha (DFFA), transcript variant 1, mRNA.
• Database Link:
  NP_004392
  Entrez Gene 1676 Human
  UniProt ID O00273
• Background: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Synonyms: DFF-45; DFF1; ICAD

Reference Data

• Protein Pathways: Apoptosis