alpha 1 Antitrypsin (SERPINA1) Mouse Monoclonal Antibody [Clone ID: OTI3C5]

CAT#: CF500069

Carrier-free (BSA/glycerol-free) SERPINA1 mouse monoclonal antibody, clone OTI3C5 (formerly 3C5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (RC202082, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1. Positive lysates LY400112 (100ug) and LC400112 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.

Specifications

Product Data

• Clone Name: OTI3C5
• Applications: WB
• Recommended Dilution: WB 1:1000~2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 26-418 of human A1AT (NP_000286) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.6 kDa
• Gene Name: Homo sapiens serpin family A member 1 (SERPINA1), transcript variant 1, mRNA.
• Database Link:
  NP_000286
  Entrez Gene 5265 Human
  UniProt ID P01009
• Background: A1AT is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in A1AT gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.
• Synonyms: A1A; A1AT; AAT; alpha1AT; PI; PI1; PRO2275

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Complement and coagulation cascades