FOXA2 Mouse Monoclonal Antibody [Clone ID: OTI3C10]

CAT#: CF500073

Carrier-free (BSA/glycerol-free) FOXA2 mouse monoclonal antibody, clone OTI3C10 (formerly 3C10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXA2 (RC211408, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXA2. Positive lysates LY407005 (100ug) and LC407005 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-FOXA2 monoclonal antibody at 1:200 dilution.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-FOXA2 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of liver using anti-FOXA2 (TA500073) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

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  Immunohistochemical staining of paraffin-embedded lung within the normal limits using anti-FOXA2 (TA500073) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

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  Anti-FOXA2 mouse monoclonal antibody (TA500073) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY FOXA2 (RC211408)

• Immunofluorescent staining of HepG2 cells using anti-FOXA2 mouse monoclonal antibody (TA500073) at 1:50 dilution.

• HEK293T cells transfected with either pCMV6-ENTRY FOXA2 (RC211408) (Red) or empty vector control plasmid (Blue) were immunostained with anti-FOXA2 mouse monoclonal (TA500073, Dilution 1:1,000), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3C10
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:1000; IHC: 1:50; IF: 1:50-1:100; Flow 1:1,000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 300-458 of human FOXA2
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48.1 kDa
• Gene Name: Homo sapiens forkhead box A2 (FOXA2), transcript variant 2, mRNA.
• Database Link:
  NP_710141
  Entrez Gene 15376 MouseEntrez Gene 25099 RatEntrez Gene 3170 Human
  UniProt ID Q9Y261
• Background: FOXA2 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene
• Synonyms: HNF3B; TCF3B

Reference Data

• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young