MAPK6 Mouse Monoclonal Antibody [Clone ID: OTI5E1]

CAT#: CF500077

Carrier-free (BSA/glycerol-free) ERK3 mouse monoclonal antibody, clone OTI5E1 (formerly 5E1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6 (RC201597, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6. Positive lysates LY400969 (100ug) and LC400969 (20ug) can be purchased separately from OriGene.

• 

  Immunofluorescent staining of HeLa cells using anti-MAPK6 mouse monoclonal antibody (TA500077).

• 

  Anti-MAPK6 mouse monoclonal antibody (TA500077) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY MAPK6 (RC201597)

• 

  Western blot analysis of extracts (10ug) from 1 cell line by using anti-MAPK6 monoclonal antibody at 1:200 dilution.

Specifications

Product Data

• Clone Name: OTI5E1
• Applications: IF, WB
• Recommended Dilution: WB: 1:1000, IF (1:100)
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 345-721 of human ERK3
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 82.5 kDa
• Gene Name: Homo sapiens mitogen-activated protein kinase 6 (MAPK6), mRNA.
• Database Link:
  NP_002739
  Entrez Gene 50772 MouseEntrez Gene 58840 RatEntrez Gene 5597 Human
  UniProt ID Q16659
• Background: ERK3 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters
• Synonyms: ERK3; HsT17250; p97MAPK; PRKM6

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase