SOX17 Mouse Monoclonal Antibody [Clone ID: OTI2G8]

CAT#: CF500096

Carrier-free (BSA/glycerol-free) Sox17 mouse monoclonal antibody, clone OTI2G8 (formerly 2G8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX17 (RC220888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX17. Positive lysates LY411669 (100ug) and LC411669 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SOX17 monoclonal antibody.

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  Figure from citation: Corresponding conventional and immunhistochemical stains of human seminoma and embryonal carcinoma by using anti-SOX17 antibody. Seminoma: positivity for SOX17 (Left); Embryonal carcinoma: negative staining for SOX17 (Right). Dilution: 1:2000 View Citation

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  Anti-SOX17 mouse monoclonal antibody (TA500096) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOX17 (RC220888).

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  Immunofluorescent staining of A549 cells using anti-SOX17 mouse monoclonal antibody (TA500096).

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Specifications

Product Data

• Clone Name: OTI2G8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:50, IF 1:50~100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 177-414 of human SOX17 (NP_071899) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.9 kDa
• Gene Name: Homo sapiens SRY-box transcription factor 17 (SOX17), mRNA.
• Database Link:
  NP_071899
  Entrez Gene 20671 MouseEntrez Gene 312936 RatEntrez Gene 486955 DogEntrez Gene 64321 Human
  UniProt ID Q9H6I2
• Background: SOX17 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. It may act as a transcriptional regulator after forming a protein complex with other proteins.
• Synonyms: VUR3

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Wnt signaling pathway