CD31 (PECAM1) Mouse Monoclonal Antibody [Clone ID: OTI8A9]

CAT#: CF500121

Carrier-free (BSA/glycerol-free) PECAM1 mouse monoclonal antibody, clone OTI8A9 (formerly 8A9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.

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  Immunohistochemical staining of paraffin-embedded lung within the normal limits using anti-PECAM1 ([TA000121]) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Specifications

Product Data

• Clone Name: OTI8A9
• Applications: IHC, WB
• Recommended Dilution: WB: 1:500, IHC 1:50
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 425-591 of human PECAM1
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 82.4 kDa
• Gene Name: Homo sapiens platelet and endothelial cell adhesion molecule 1 (PECAM1)
• Database Link:
  NP_000433
  Entrez Gene 5175 Human
  UniProt ID P16284
• Background: The protein encoded by this gene is a cell adhesion molecule expressed on platelets and at endothelial cellintercellular junctions
• Synonyms: CD31; EndoCAM; endoCAM; GPIIA; PECA1; PECAM-1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration