EPCAM Mouse Monoclonal Antibody [Clone ID: OTI2C8]

CAT#: CF500171

Carrier-free (BSA/glycerol-free) EpCAM mouse monoclonal antibody, clone OTI2C8 (formerly 2C8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TACSTD1 (RC201989, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TACSTD1. Positive lysates LY400847 (100ug) and LC400847 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TACSTD1 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TACSTD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500171)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TACSTD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500171)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TACSTD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500171)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TACSTD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500171)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TACSTD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500171)

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Specifications

Product Data

• Clone Name: OTI2C8
• Applications: IHC, WB
• Recommended Dilution: WB 1:200~500, IHC 1:50
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 24-265 of human EpCAM (NP_002345) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.7 kDa
• Gene Name: Homo sapiens epithelial cell adhesion molecule (EPCAM), mRNA.
• Database Link:
  NP_002345
  Entrez Gene 4072 Human
  UniProt ID P16422
• Background: EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas.
• Synonyms: 4; DIAR5; EGP-2; EGP40; EGP314; ESA; HNPCC8; KS1; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transmembrane