Oncostatin M (OSM) Mouse Monoclonal Antibody [Clone ID: OTI2B6]

CAT#: CF500195

Carrier-free (BSA/glycerol-free) OSM mouse monoclonal antibody, clone OTI2B6 (formerly 2B6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSM (RC204277, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSM. Positive lysates LY412433 (100ug) and LC412433 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-OSMmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500195, Dilution 1:50)

Specifications

Product Data

• Clone Name: OTI2B6
• Applications: IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 25-252 of human OSM
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.7 kDa
• Gene Name: Homo sapiens oncostatin M (OSM), transcript variant 1, mRNA.
• Database Link:
  NP_065391
  Entrez Gene 5008 Human
  UniProt ID P13725
• Background: Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells
• Synonyms: MGC20461

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - DSL/Notch pathway, Stem cell relevant signaling - JAK/STAT signaling pathway
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway