Flt3 ligand (FLT3LG) Mouse Monoclonal Antibody [Clone ID: OTI8D10]

CAT#: CF500274

Carrier-free (BSA/glycerol-free) FLT3LG mouse monoclonal antibody, clone OTI8D10 (formerly 8D10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FLT3LG (RC222242, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FLT3LG. Positive lysates LY419927 (100ug) and LC419927 (20ug) can be purchased separately from OriGene.

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  Anti-FLT3LG mouse monoclonal antibody (TA500274) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FLT3LG (RC222242).

Specifications

Product Data

• Clone Name: OTI8D10
• Applications: IF, WB
• Recommended Dilution: WB 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 27-181 of human FLT3LG
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.4 kDa
• Gene Name: Homo sapiens fms related tyrosine kinase 3 ligand (FLT3LG), transcript variant 3, mRNA.
• Database Link:
  NP_001450
  Entrez Gene 2323 Human
  UniProt ID P49771
• Background: Stimulates the proliferation of early hematopoietic cells. Synergizes well with a number of other colony stimulating factors and interleukins
• Synonyms: FL; FLT3L

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Pathways in cancer