Prominin 2 (PROM2) Mouse Monoclonal Antibody [Clone ID: OTI5E5]

CAT#: CF500310

Carrier-free (BSA/glycerol-free) PROM2 mouse monoclonal antibody, clone OTI5E5 (formerly 5E5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PROM2 (RC211605, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROM2. Positive lysates LY403405 (100ug) and LC403405 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-PROM2 monoclonal antibody (1:200).

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  Anti-PROM2 mouse monoclonal antibody (TA500310) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PROM2 (RC211605).

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  Immunoprecipitation (IP) of PROM2 by using TrueMab monoclonal anti-PROM2 antibodies (Negative control: IP without adding anti-PROM2 antibody.). For each experiment, 500ul of DDK tagged PROM2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PROM2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Specifications

Product Data

• Clone Name: OTI5E5
• Applications: IF, IP, WB
• Recommended Dilution: WB 1:2000, IP: 4ug/mL
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human PROM2 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 91.8 kDa
• Gene Name: Homo sapiens prominin 2 (PROM2), transcript variant 3, mRNA.
• Database Link:
  NP_653308
  Entrez Gene 150696 Human
  UniProt ID Q8N271
• Synonyms: PROML2

Reference Data

• Protein Families: Transmembrane