TRPM4 Mouse Monoclonal Antibody [Clone ID: OTI10H5]

CAT#: CF500381

Carrier-free (BSA/glycerol-free) TRPM4 mouse monoclonal antibody, clone OTI10H5 (formerly 10H5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRPM4 (RC216888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRPM4. Positive lysates LY413636 (100ug) and LC413636 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRPM4 monoclonal antibody.

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  Figure from citation: Western Blot of TRPM4 protein level by using anti-TRPM4 antibody in human endothelial cells. View Citation

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500381)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500381)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500381)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500381)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TRPM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500381)

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Specifications

Product Data

• Clone Name: OTI10H5
• Applications: IHC, WB
• Recommended Dilution: WB 1:5000~10000, IHC 1:50
• Reactivities: Human, Monkey, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TRPM4 (NP_060106) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 134.3 kDa
• Gene Name: Homo sapiens transient receptor potential cation channel subfamily M member 4 (TRPM4), transcript variant 1, mRNA.
• Database Link:
  NP_060106
  Entrez Gene 484385 DogEntrez Gene 719120 MonkeyEntrez Gene 54795 Human
  UniProt ID Q8TD43
• Background: Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload.
• Synonyms: hTRPM4; LTrpC4; PFHB1B; TRPM4B

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane