TrkB (NTRK2) Mouse Monoclonal Antibody [Clone ID: OTI2E1]

CAT#: CF500386

Carrier-free (BSA/glycerol-free) NTRK2 mouse monoclonal antibody, clone OTI2E1 (formerly 2E1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NTRK2 (Cat# RC205129, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTRK2(Cat# TA500386). Positive lysates LY400391 (100ug) and LC400391 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-NTRK2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Anti-NTRK2 mouse monoclonal antibody (TA500386) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NTRK2 (RC205129).

Specifications

Product Data

• Clone Name: OTI2E1
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NTRK2 (NP_001007098) produced in HEK293T cell
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 92 kDa
• Gene Name: neurotrophic receptor tyrosine kinase 2
• Database Link:
  NP_001007098
  Entrez Gene 18212 MouseEntrez Gene 25054 RatEntrez Gene 4915 Human
  UniProt ID Q16620
• Background: This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternate transcriptional splice variants encoding different isoforms have been found for this gene
• Synonyms: GP145-TrkB; trk-B; TRKB

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: MAPK signaling pathway, Neurotrophin signaling pathway