SSB Mouse Monoclonal Antibody [Clone ID: OTI1E11]

CAT#: CF500406

Carrier-free (BSA/glycerol-free) SSB mouse monoclonal antibody, clone OTI1E11 (formerly 1E11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (RC205013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB. Positive lysates LY401091 (100ug) and LC401091 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-SSB monoclonal antibody (1:200).

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SSB monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Anti-SSB mouse monoclonal antibody (TA500406) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB (RC205013).

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  Immunofluorescent staining of HepG2 cells using anti-SSB mouse monoclonal antibody (TA500406).

• Immunoprecipitation (IP) of SSB by using TrueMab monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SSB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

• Figure from citation: Immunoprecipitation of SSB (La) protein level by using anti-SSB antibody in HeLa cells. View Citation

• Flow cytometric Analysis of Jurkat cells, using anti-SSB antibody (TA500406), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1E11
• Applications: FC, IF, IP, WB
• Recommended Dilution: WB 1:1000~2000, IF 1:50~100, FLOW 1:100, IP 2ug/500ul
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human SSB expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.8 kDa
• Gene Name: Homo sapiens small RNA binding exonuclease protection factor La (SSB), transcript variant 1, mRNA.
• Database Link:
  NP_003133
  Entrez Gene 20823 MouseEntrez Gene 81783 RatEntrez Gene 478787 DogEntrez Gene 710344 MonkeyEntrez Gene 6741 Human
  UniProt ID P05455
• Background: La is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(H) elements of newly RNA polymerase III (see MIM 606007)-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAsassociated with hepatitis C virus. La protein was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome (MIM 270150) and systemic lupus erythematosus
• Synonyms: La; LARP3; SSB

Reference Data

• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Systemic lupus erythematosus