MEK4 (MAP2K4) Mouse Monoclonal Antibody [Clone ID: OTI2E4]

CAT#: CF500412

Carrier-free (BSA/glycerol-free) MAP2K4 mouse monoclonal antibody, clone OTI2E4 (formerly 2E4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (RC206051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4. Positive lysates LY401058 (100ug) and LC401058 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500412, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-MAP2K4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500412, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-MAP2K4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500412, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-MAP2K4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500412, Dilution 1:50)

• 

  Anti-MAP2K4 mouse monoclonal antibody (TA500412) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K4 (RC206051).

Show all 6 images

Specifications

Product Data

• Clone Name: OTI2E4
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000 IHC 1:50
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human MAP2K4 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.3 kDa
• Gene Name: Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4), transcript variant 1, mRNA.
• Database Link:
  NP_003001
  Entrez Gene 26398 MouseEntrez Gene 287398 RatEntrez Gene 6416 Human
  UniProt ID P45985
• Background: This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
• Synonyms: JNKK; JNKK1; MAPKK4; MEK4; MKK4; PRKMK4; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway