TYRO3 Mouse Monoclonal Antibody [Clone ID: OTI2D11]

CAT#: CF500417

Carrier-free (BSA/glycerol-free) TYRO3 mouse monoclonal antibody, clone OTI2D11 (formerly 2D11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (RC208260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3. Positive lysates LY401899 (100ug) and LC401899 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500417)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500417)

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  Anti-TYRO3 mouse monoclonal antibody (TA500417) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TYRO3 (RC208260).

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  Immunoprecipitation (IP) of TYRO3 by using TrueMab monoclonal anti-TYRO3 antibodies (Negative control: IP without adding anti-TYRO3 antibody.). For each experiment, 500ul of DDK tagged TYRO3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-TYRO3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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Specifications

Product Data

• Clone Name: OTI2D11
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, IP 2ug/500ul
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TYRO3 (NP_006284) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 96.7 kDa
• Gene Name: Homo sapiens TYRO3 protein tyrosine kinase (TYRO3), transcript variant 1, mRNA.
• Database Link:
  NP_006284
  Entrez Gene 22174 MouseEntrez Gene 25232 RatEntrez Gene 7301 Human
  UniProt ID Q06418
• Background: The gene is part of a 3-member transmembrane receptor kinase receptor family with a processed pseudogene distal on chromosome 15. The encoded protein is activated by the products of the growth arrest-specific gene 6 and protein S genes and is involved in controlling cell survival and proliferation, spermatogenesis, immunoregulation and phagocytosis. The encoded protein has also been identified as a cell entry factor for Ebola and Marburg viruses. [provided by RefSeq]
• Synonyms: BYK; Dtk; Etk-2; Rek; RSE; Sky; Tif

Reference Data

• Protein Families: Druggable Genome, Protein Kinase