TYRO3 Mouse Monoclonal Antibody [Clone ID: OTI9B12]

CAT#: CF500421

Carrier-free (BSA/glycerol-free) TYRO3 mouse monoclonal antibody, clone OTI9B12 (formerly 9B12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TYRO3 (RC208260, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TYRO3. Positive lysates LY401899 (100ug) and LC401899 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TYRO3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TYRO3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Anti-TYRO3 mouse monoclonal antibody (TA500421) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TYRO3 (RC208260).

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  Immunoprecipitation (IP) of TYRO3 by using TrueMab monoclonal anti-TYRO3 antibodies (Negative control: IP without adding anti-TYRO3 antibody.). For each experiment, 500ul of DDK tagged TYRO3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-TYRO3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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  HEK293T cells transfected with either RC208260 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TYRO3 antibody (TA500421), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI9B12
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100, IP 2ug/500ul
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TYRO3 (NP_006284) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 96.9 kDa
• Gene Name: TYRO3 protein tyrosine kinase
• Database Link:
  NP_006284
  Entrez Gene 22174 MouseEntrez Gene 25232 RatEntrez Gene 7301 Human
  UniProt ID Q06418
• Background: May be involved in cell adhesion processes, particularly in the central nervous system.In case of filovirus infection, seems to function as a cell entry factor.
• Synonyms: BYK; Dtk; Etk-2; Rek; RSE; Sky; Tif

Reference Data

• Protein Families: Druggable Genome, Protein Kinase