Antithrombin III (SERPINC1) Mouse Monoclonal Antibody [Clone ID: OTI8D5]

CAT#: CF500455

Carrier-free (BSA/glycerol-free) SERPINC1 mouse monoclonal antibody, clone OTI8D5 (formerly 8D5)

Product Images

HEK293T cells transfected with either RC222845 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SERPINC1 antibody (TA500455), and then analyzed by flow cytometry.

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SERPINC1 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Specifications

Product Data

• Clone Name: OTI8D5
• Applications: FC, WB
• Recommended Dilution: FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SERPINC1 (NP_000479) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.6 kDa
• Gene Name: Homo sapiens serpin family C member 1 (SERPINC1), transcript variant 1, mRNA.
• Database Link:
  NP_000479
  Entrez Gene 11905 MouseEntrez Gene 304917 RatEntrez Gene 462 Human
  UniProt ID P01008
• Background: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
• Synonyms: AT3; AT3D; ATIII; THPH7

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades