CD4 Mouse Monoclonal Antibody [Clone ID: OTI18E3]

CAT#: CF500483

Carrier-free (BSA/glycerol-free) CD4 mouse monoclonal antibody, clone OTI18E3 (formerly 18E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD4 (RC206453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD4. Positive lysates LY400209 (100ug) and LC400209 (20ug) can be purchased separately from OriGene.

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  Flow cytometric Analysis of live Jurkat cells, using anti-CD4 antibody (TA500483 at 1:100, Red), compared to IgG1 isotype control (Black).

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  Flow cytometric Analysis of living Molt-4 cells, using anti-CD4 antibody (TA500483) (Red), compared PBS (Blue).

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  Flow cytometric Analysis of living RBC-lysed human peripheral blood cells, using anti-CD4 antibody (TA500483), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

Specifications

Product Data

• Clone Name: OTI18E3
• Applications: FC, WB
• Recommended Dilution: WB 1:1000~2000, FC 1:100
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD4 (NP_000607) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.1 kDa
• Gene Name: Homo sapiens CD4 molecule (CD4), transcript variant 1, mRNA.
• Database Link:
  NP_000607
  Entrez Gene 24932 RatEntrez Gene 920 Human
  UniProt ID P01730
• Background: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
• Synonyms: CD4mut

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane
• Protein Pathways: Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway