Superoxide Dismutase 1 (SOD1) Mouse Monoclonal Antibody [Clone ID: OTI8B10]

CAT#: CF500495

Carrier-free (BSA/glycerol-free) SOD1 mouse monoclonal antibody, clone OTI8B10 (formerly 8B10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOD1 (RC200725, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOD1. Positive lysates LY400160 (100ug) and LC400160 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 6 different cell lines by using anti-SOD1 monoclonal antibody (HepG2: human; HeLa: human; HT29: human; SVT2: mouse; A549: human; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500495)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500495)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500495)

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  HEK293T cells transfected with either RC200725 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SOD1 antibody (TA500495), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI8B10
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:50, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SOD1 (NP_000445) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 15.8 kDa
• Gene Name: Homo sapiens superoxide dismutase 1 (SOD1), mRNA.
• Database Link:
  NP_000445
  Entrez Gene 6647 Human
  UniProt ID P00441
• Background: The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq]
• Synonyms: ALS; ALS1; HEL-S-44; homodimer; hSod1; IPOA; SOD

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Huntington's disease, Prion diseases