STK39 Mouse Monoclonal Antibody [Clone ID: OTI4E3]

CAT#: CF500501

Carrier-free (BSA/glycerol-free) STK39 mouse monoclonal antibody, clone OTI4E3 (formerly 4E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STK39 (RC223981, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STK39. Positive lysates LY402227 (100ug) and LC402227 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-STK39 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-STK39 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500501)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-STK39 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500501)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-STK39 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500501)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-STK39 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500501)

• Immunofluorescent staining of COS7 cells using anti-STK39 mouse monoclonal antibody (TA500501).

• HEK293T cells transfected with either RC223981 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-STK39 antibody (TA500501), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI4E3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:50, IF 1:50, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human STK39 (NP_037365) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 59.3 kDa
• Gene Name: Homo sapiens serine/threonine kinase 39 (STK39), mRNA.
• Database Link:
  NP_037365
  Entrez Gene 53416 MouseEntrez Gene 54348 RatEntrez Gene 27347 Human
  UniProt ID Q9UEW8
• Background: This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress. [provided by RefSeq]
• Synonyms: DCHT; PASK; SPAK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase