TMEM100 Mouse Monoclonal Antibody [Clone ID: OTI1F8]

CAT#: CF500508

Carrier-free (BSA/glycerol-free) TMEM100 mouse monoclonal antibody, clone OTI1F8 (formerly 1F8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMEM100 (RC203980, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMEM100. Positive lysates LY402665 (100ug) and LC402665 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TMEM100 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-TMEM100 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500508, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-TMEM100mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500508, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-TMEM100mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500508, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-TMEM100mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500508, Dilution 1:50)

• Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-TMEM100mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500508, Dilution 1:50)

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Specifications

Product Data

• Clone Name: OTI1F8
• Applications: IHC, WB
• Recommended Dilution: WB 1:1000 IHC 1:50
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human TMEM100 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14.4 kDa
• Gene Name: Homo sapiens transmembrane protein 100 (TMEM100), transcript variant 2, mRNA.
• Database Link:
  NP_060756
  Entrez Gene 609661 DogEntrez Gene 707032 MonkeyEntrez Gene 55273 Human
  UniProt ID Q9NV29
• Synonyms: FLJ10970; FLJ37856

Reference Data

• Protein Families: Transmembrane