NEK6 Mouse Monoclonal Antibody [Clone ID: OTI8G5]

CAT#: CF500511

Carrier-free (BSA/glycerol-free) NEK6 mouse monoclonal antibody, clone OTI8G5 (formerly 8G5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (RC203609, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6. Positive lysates LY402327 (100ug) and LC402327 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500511, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-NEK6mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500511, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-NEK6mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500511, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-NEK6mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500511, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-NEK6mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500511, Dilution 1:50)

• Anti-NEK6 mouse monoclonal antibody (TA500511) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK6 (RC203609).

• Immunoprecipitation of NEK6 by using TrueMab monoclonal anti-NEK6 antibody (Negative control: IP without adding anti-NEK6 antibody). For each experiment, 500ul of DDK tagged NEK6 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEK6 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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Specifications

Product Data

• Clone Name: OTI8G5
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, Flow 1:500, IP 2-4ug/mg
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human NEK6 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.7 kDa
• Gene Name: Homo sapiens NIMA related kinase 6 (NEK6), transcript variant 2, mRNA.
• Database Link:
  NP_055212
  Entrez Gene 59126 MouseEntrez Gene 360161 RatEntrez Gene 10783 Human
  UniProt ID Q9HC98
• Background: The Aspergillus nidulans 'never in mitosis A' (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA
• Synonyms: SID6-1512

Reference Data

• Protein Families: Druggable Genome, Protein Kinase