LAT2 (SLC7A8) Mouse Monoclonal Antibody [Clone ID: OTI8B12]

CAT#: CF500513

Carrier-free (BSA/glycerol-free) SLC7A8 mouse monoclonal antibody, clone OTI8B12 (formerly 8B12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC7A8 (RC208586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC7A8. Positive lysates LY402177 (100ug) and LC402177 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-SLC7A8 monoclonal antibody at 1:200 dilution.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-SLC7A8mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500513, Dilution 1:50)

Specifications

Product Data

• Clone Name: OTI8B12
• Applications: IHC, WB
• Recommended Dilution: WB 1:500 IHC 1:50 Flow 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human SLC7A8 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58.4 kDa
• Gene Name: Homo sapiens solute carrier family 7 member 8 (SLC7A8), transcript variant 1, mRNA.
• Database Link:
  NP_036376
  Entrez Gene 50934 MouseEntrez Gene 84551 RatEntrez Gene 23428 Human
  UniProt ID Q9UHI5
• Background: Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine orD,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney
• Synonyms: LAT2; LPI-PC1

Reference Data

• Protein Families: Druggable Genome, Transmembrane