FRK Mouse Monoclonal Antibody [Clone ID: OTI5G4]

CAT#: CF500525

Carrier-free (BSA/glycerol-free) FRK mouse monoclonal antibody, clone OTI5G4 (formerly 5G4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (RC204460, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FRK. Positive lysates LY400740 (100ug) and LC400740 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FRK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500525)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FRK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500525)

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  Anti-FRK mouse monoclonal antibody (TA500525) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FRK (RC204460).

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  HEK293T cells transfected with either RC204460 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FRK antibody (TA500525), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5G4
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FRK (NP_002022) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58.1 kDa
• Gene Name: Homo sapiens fyn related Src family tyrosine kinase (FRK), mRNA.
• Database Link:
  NP_002022
  Entrez Gene 14302 MouseEntrez Gene 79209 RatEntrez Gene 2444 Human
  UniProt ID P42685
• Background: The protein encoded by this gene belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth. [provided by RefSeq]
• Synonyms: GTK; PTK5; RAK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase