TMEM100 Mouse Monoclonal Antibody [Clone ID: OTI10G3]

CAT#: CF500532

Carrier-free (BSA/glycerol-free) TMEM100 mouse monoclonal antibody, clone OTI10G3 (formerly 10G3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMEM100 (RC203980, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMEM100. Positive lysates LY402665 (100ug) and LC402665 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TMEM100 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500532)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TMEM100 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500532)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TMEM100 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500532)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TMEM100 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500532)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TMEM100 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500532)

• Anti-TMEM100 mouse monoclonal antibody (TA500532) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TMEM100 (RC203980).

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Specifications

Product Data

• Clone Name: OTI10G3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TMEM100 (NP_060756) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14.4 kDa
• Gene Name: Homo sapiens transmembrane protein 100 (TMEM100), transcript variant 2, mRNA.
• Database Link:
  NP_060756
  Entrez Gene 55273 Human
  UniProt ID Q9NV29
• Synonyms: FLJ10970; FLJ37856

Reference Data

• Protein Families: Transmembrane